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A single-cylinder engine was operated in HCCI combustion mode with different kinds of commercial fuels. The HCCI combustion was generated by creating a negative valve overlap (early exhaust valve closing combined with late intake valve opening) thus trapping a large amount of residuals (∼ 55%). Fifteen different fuels with high octane numbers were tested six of which were primary reference fuels (PRF's) and nine were commercial fuels or reference fuels. The engine was operated at constant operational parameters (speed/load, valve timing and equivalence ratio, intake air temperature, compression ratio, etc.) changing only the fuel type while the engine was running. Changing the fuel affected the auto-ignition timing, represented by the 50% mass fraction burned location (CA50). However these changes were not consistent with the classical RON and MON numbers, which are measures of the knock resistance of the fuel. Indeed, no correlation was found between CA50 and the RON or MON numbers.