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Alcohol-based fuels are a viable alternative to fossil fuels for powering vehicles. As a drop-in fuel, an oxygenated fuel blend containing the C8 alcohol 2-ethylhexanol (isomer of octanol), hydrotreated vegetable oil (HVO) and rapeseed methyl ester (RME) can reduce soot and NOx emissions whilst maintaining engine performance. However, fuel injection strategy significantly affects combustion and hence has been investigated with a view to reducing emissions whilst maintaining engine efficiency. In a single cylinder light-duty compression ignition research engine, the effect of different injection strategies (main, main/post, double pre/main, double pre/main/post injection) and EGR levels (0%, 19%) on specifically NOx, soot emissions and particle size distribution was investigated for three different fuels: fossil diesel fuel, HVO and the oxygenated blend. The blend was designed to have diesel-like combustion properties (cetane number of 52) and had an oxygen content of 5.4% by mass.