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Three replicate aerobic-heterotrophic biotrickling filters were designed to promote the simultaneous biodegradation of graywater and a waste gas containing NH3, H2S and CO2. Upon visual observation of discolored solids, it was originally hypothesized that gas-phase CO2 concentrations were excessive, causing regions of anoxic zones to form within the biotrickling filters. Observed discolored (black) biofilm of this nature is typically assumed to be either lysed bacterial cells or anaerobic regions, implying alteration of operational conditions. Solid (biofilm) samples were collected in the presence and absence of gas-phase wastestream(s) to determine if the gas-phase contaminants were contributing to the solid-phase discoloration. Two sets of experiments (shaker flask and solids characterization) were conduced to determine the nature of the discolored solids. Results indicated that the discolored solids were neither anaerobic bacteria nor lysed cells.

Most of the gaseous contaminants generated inside ALS (Advanced Life Support) cabins can be degraded to some degree by microbial degradation in a biofilter. The entry of biofiltration techniques into ALS will most likely involve integration with existing physico-chemical methods. However, in this study, cabin air quality treated by only biofiltration was predicted using the one-box and biofiltration models. Based on BVAD (Baseline Values and Assumptions Document) and SMAC (Spacecraft Maximum Allowable Concentrations), ammonia and carbon monoxide will be the critical compounds for biofilter design and control. Experimentation is needed to identify the pertinent microbial parameters and removal efficiency of carbon monoxide and to validate the results of this preliminary investigation.

The Bioregenerative Air Treatment for Health system has been proposed for Advanced Life Support (ALS) planetary base applications. The system will be operated as a biotrickling filter to simultaneously treat graywater and waste gas. Preliminary experiments have focused on carbon removal from a graywater simulant. Six bench scale biotrickling filter reactors were constructed and monitored continuously. After a reactor startup phase of 40 days, the average total organic carbon (TOC) removal for reactors packed with Tri-packs® packing material was 62%. A second set of experiments was designed to evaluate TOC removal using different packing materials (Bee-cell and Biobale). It was hypothesized that the alternative packing materials would reduce the effects of channeling in the reactors, thus improving TOC removal. However, TOC removal did not significantly improve during the second set of experiments.

Resource recovery, including that of urine water extraction, is one of the most crucial aspects of long-term life support in interplanetary space travel. This paper will consequently examine an innovative approach to processing raw, undiluted urine based on low-temperature freezing. This strategy is uniquely different from NASA's current emphasis on either ‘integrated’ (co-treatment of mixed urine, grey, and condensate waters) or ‘high-temperature’ (i.e., VCD [vapor compression distillation] or VPCAR [vapor phase catalytic ammonia removal]) processing strategies, whereby this liquid freeze-thaw (LiFT) procedure would avoid both chemical and microbial cross-contamination concerns while at the same time securing highly desirable reductions in likely ESM levels.

The application of biological treatment to solid waste is very promising to facilitate recycling of water, carbon, and nutrients and to reduce the resupply needs of long-term crewed space missions. Degradation of biodegradable solid wastes generated during such a mission is under investigation as part of the NASA Center of Research and Training (NSCORT) at Purdue University. Processing in the solids thermophilic aerobic reactor (STAR) involves the use of high temperature micro-aerobic slurry conditions to degrade solid wastes, enabling the recycling of water, carbon, and nutrients for further downstream uses. Related research presently underway includes technical development and optimization of STAR operations as well as a complementary evaluation of post-STAR processing for gas-stream purification, water recovery by condensate purification, and residuals utilization for both mushroom growth media and nutritional support for fish growth.

Complete reuse of graywater will be essential during long duration human space missions. The highest loaded and most important component to remove from graywater is surfactant, the active ingredient in soaps and detergents. When considering a biological treatment system for processing of graywater, surfactant biodegradability becomes a very important consideration. Surfactants should be chosen that are degraded at a fast rate and yield inconsequential degradation byproducts. Experiments conducted for this research examined the biodegradation of the surfactants in Pert Plus for Kids, disodium cocoamphodiacetate (DSCADA) and sodium laureth-3 sulfate (SLES), using respirometry. Rates of CO2 production, or ultimate degradation, are reported. DSCADA was found to be toxic to bacteria when present at 270 ppm whereas no toxicity was observed during experiments with SLES.