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To meet the stringent emission regulations on diesel engines, engine-out emissions have been lowered by adapting new combustion concepts such as low-temperature combustion and after-treatment systems have also been used to reduce tailpipe emissions. To optimize the control of both in-cylinder combustion and the efficiency of an after treatment system to reduce NOx, the amount of real-time NOx emissions should be determined. Therefore, in previous studies, the authors developed a real-time NO estimation model based on the in-cylinder pressure and the data available from the ECU during engine operation. The model was evaluated by comparing its results with a CFD model, which agreed well. Then, the model was implemented on an embedded system which allows real-time applications, and was verified on a 2.2-liter diesel engine. The model showed good agreement with the experimental results at various steady-state conditions and simple transient conditions.