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The impact of lubricant sulphur and phosphorus levels on the formation of nucleation mode particles was explored in a light duty diesel vehicle operating over the New European Drive Cycle (NEDC). All measurements were undertaken using a Scanning Mobility Particle Sizer (SMPS), sampling from a conventional Constant Volume Sampler (CVS) system. Rigorous sampling system and vehicle conditioning procedures were applied to eliminate oil carry-over and nanoparticle artifact formation. An initial vehicle selection process was undertaken on vehicles representing three fuel injection strategies, namely; distributor pump, common rail and unit injector. The vehicles met Euro III specifications and were all equipped with oxidation catalysts. Idle and low load stability were key requirements, since these conditions are the most significant in terms of their propensity to generate nucleation mode particles.