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The operation of a crop growth system in micro-gravity is an important part of the National Aeronautics and Space Administration's Closed Ecological Life Support System development program. Maintaining densely arrayed plants in a closed environment imposed to induce high growth rates must be expected to result in substantial levels of water transpiration rate. Since the environmental air is recirculated, the transpiration water must be removed. In an operating CELSS, it is expected that this water will provide potable water for use of the crew. There is already considerable knowledge about water removal from crew environmental air during orbital and transfer activities, and the difference between the conditions of the described requirement and the conditions for which experience has been gained is the quantities involved and the reliability implications due to the required periods of operation.

We present a device for collecting and storing feces in microgravity that is user-friendly yet suitable for spacecraft in which cabin volume and mass are constrained. On Apollo missions, the commode function was served using disposable plastic bags, which proved time-consuming and caused odor problems. On Skylab, the space shuttle, and the International Space Station, toilets have used airflow beneath a seat to control odors and collect feces. We propose to incorporate airflow into a system of self-compacting, self-drying collection and stowage bags, providing the benefits of previous commodes while minimizing mass and volume. Each collection bag consists of an inner layer of hydrophobic membrane that is permeable to air but not liquid or solid waste, an outer layer of impermeable plastic, and a collapsible spacer separating the inner and outer layers. Filled bags are connected to space vacuum, compacting and drying their contents.

The initial concepts and construction of a three layered, water-absorbent, zero-G, compactor trash bag will be described. This bag is composed of an inner wicking layer, a middle absorbent layer, and an outer containment layer. The primary properties of the wicking layer are the fast adsorption of any free liquid released within the trash bag and the lateral spreading of this liquid around the interior of the bag. The absorbent layer sequesters and stores the liquid captured by the wicking layer. It need not be as fast acting as the wicking layer, but has to have a much larger capacity. The containment layer allows for handling of the bag without worry of releasing the contents. The combined strength of the three layers needs to be sufficient to withstand the forces exerted by the compactor.

This paper considers the design of a life support system for transit to Mars and return to Earth. Because of the extremely high cost of launching mass to Mars, the Mars transit life support system must minimize the amount of oxygen, water, and food transported. The three basic ways to provide life support are to directly supply all oxygen and water, or to recycle them using physicochemical equipment, or to produce them incidentally while growing food using crop plants. Comparing the costs of these three approaches shows that physicochemical recycling of oxygen and water is least costly for a Mars transit mission. The long mission duration also requires that the Mars transit life support system have high reliability and maintainability. Mars transit life support cannot make use of planetary resources or gravity. It should be tested in space on the International Space Station (ISS).

A habitat for housing up to 32 Tenebrionid, black body beetles (Trigonoscelis gigas Reitter) has been developed at Ames Research Center for conducting studies to evaluate the effects of long duration spaceflight upon insect circadian timing systems. This habitat, identified as the Beetle Kit, provides an automatically controlled lighting system and activity and temperature recording devices, as well as individual beetle enclosures. Each of the 32 enclosures in a Beetle Kit allows for ad lib movement of the beetle as well as ventilation of the beetle enclosure via an externally operated hand pump. Two Beetle Kits were launched on STS-84 (Shuttle-Mir Mission-06) on May 15, 1997 and were transferred to the Priroda module of the Russian Mir space station on May 18 as part of the NASA/Mir Phase 1 Science Program. Following the Progress collision with Spektr on June 25, the Kits were transferred to the Kristall module. The beetles will remain on Mir for approximately 135 days.

This study examines the current state of the art in rodent habitats as well as the next generation of rodent habitats currently under development at NASAs Ames Research Center. Space Shuttle missions are currently limited in duration to just over two weeks. In contrast to this, future life science missions aboard the Space Station may last months or even years. This will make resource conservation and utilization critical issues in the development of rodent habitats for extended microgravity missions. Emphasis is placed on defining rodent requirements for extended space flights of up to 90 days, and on improving habitability and extending the useful performance life of rodent habitats.

An innovative design that meets both Shuttle and Space Station requirements for a user-friendly, volume-efficient, portable glovebox system has been developed at Ames Research Center (ARC). The Standard Interface Glovebox (SIGB) has been designed as a two Middeck locker-sized system that mounts in a Middeck Rack Structure (MRS) or in any rack using the Standard Interface Rack (SIR) rail spacing. The MRS provides structural support for the SIGB during all aspects of the mission and is an interface consistent with NASA's desire for commonality of mechanical interfaces, allowing the SIGB to be flown on essentially any manned space platform. The SIGB provides an enclosed work volume which operates at negative pressure relative to ambient, as well as excellent lighting and ample work volume for anticipated life sciences-related experiment operations inflight.

The space environment presents many challenges to the operation and functioning of life support systems. These challenges include reduced gravity, near vacuum ambient, extreme temperatures, and radiation. Proper testing and modeling of system components to account for these factors will be important for their verification. This paper describes the modeling and design of a reduced gravity test rig for waste management studies. The first investigation planned relate to the functioning of components of the Flexible Membrane Commode (FMC) currently under development at NASA Ames Research Center. The planned reduced gravity tests will be carried out in NASA's C'9 aircraft which provides approximately 25 seconds of reduced gravity per parabolic trajectory. The filling of the commode bag under the influence of a directed air flow will be studied. Simulated waste will be injected and cabin air will be used for directing the waste into the bag.

The Flexible Membrane Commode (FMC) is an alternative waste management system designed to address the severe mass restrictions on the Orion vehicle. The concept includes a deployable seat and single use, three layer bags that employ air flow to draw solids away from the body and safely contain them in disposable bags.1 Simulated microgravity testing of the system was performed during two separate parabolic flight campaigns in July and August of 2008. Experimental objectives included verifying the waste fill procedures in reduced gravity, characterizing waste behavior during the filling process, and comparison of the results with model predictions. In addition the operational procedure for bag installation, removal, and sealing were assessed. 2 A difficult operational requirement concerns the delivery of the fecal waste simulant into the upper area of the bag in a manner that faithfully simulates human defecation.

Two separate experimental rigs used in tests on NASA and Zero-G Corporation aircrafts flying low-gravity trajectories, and in the NASA 2.2 Second Drop Tower have been developed to test the functioning of the Flexible Membrane Commode (FMC) concept under reduced gravity conditions. The first rig incorporates the flexible, optically opaque membrane bag and the second rig incorporates a transparent chamber with a funnel assembly for evacuation that approximates the size of the membrane bag. Different waste dispensers have been used including a caulking gun and flexible hose assembly, and an injection syringe. Waste separation mechanisms include a pair of wire cutters, an iris mechanism, as well as discrete slug injection. The experimental work is described in a companion paper. This paper focuses on the obtained results and analysis of the data.

The purification of waste water is a critical element of any long-duration space mission. The Vapor Phase Catalytic Ammonia Removal (VPCAR) system offers the promise of a technology requiring low quantities of expendable material that is suitable for exploration missions. NASA has funded an effort to produce an engineering development unit specifically targeted for integration into the NASA Johnson Space Center's Integrated Human Exploration Mission Simulation Facility (INTEGRITY) formally known in part as the Bioregenerative Planetary Life Support Test Complex (Bio-Plex) and the Advanced Water Recovery System Development Facility. The system includes a Wiped-Film Rotating-Disk (WFRD) evaporator redesigned with micro-gravity operation enhancements, which evaporates wastewater and produces water vapor with only volatile components as contaminants. Volatile contaminants, including organics and ammonia, are oxidized in a catalytic reactor while they are in the vapor phase.

Determining the fundamental role of gravity in vital biological systems in space is one of six science and research areas that provides the philosophical underpinning for why NASA exists. The study of cells, tissues, and microorganisms in a spaceflight environment holds the promise of answering multiple intriguing questions about how gravity affects living systems. To enable these studies, specimens must be maintained in an environment similar to that used in a laboratory. Cell culture studies under normal laboratory conditions involve maintaining a highly specialized environment with the necessary temperature, humidity control, nutrient, and gas exchange conditions. These same cell life support conditions must be provided by the International Space Station (ISS) Cell Culture Unit (CCU) in the unique environment of space. The CCU is a perfusion-based system that must function in microgravity, at unit gravity (1g) on earth, and from 0.1g up to 2g aboard the ISS centrifuge rotor.

The animal habitat under development for the International Space Station (ISS) provides a unique opportunity for the physiological and biological science community to perform controlled experiments in microgravity on rats and mice. This paper discusses the complexities that arise in developing a new animal habitat to be flown aboard the ISS. Such development is incremental and moves forward by employing the past successes, learning from experienced shortcomings, and utilizing the latest technologies. The standard vivarium cage on the ground can be a very simple construction, however the habitat required for rodents in microgravity on the ISS is extremely complex. This discussion presents an overview of the system requirements and focuses on the unique scientific and engineering considerations in the development of the controlled animal habitat parameters. In addition, the challenges to development, specific science, animal welfare, and engineering issues are covered.

Crop production in space habitats is currently under consideration as part of an advanced life support system. The scenarios for crop production vary depending on the mission objectives. For a mission scenario such as the International Space Station (ISS), current efforts propose only salad crop production. However in order to grow salad crops, there is a need for plant nutrients (elements) such as N, P, K, Ca, etc., which constitutes about 10% of dry weight of the plant. Nitrogen and potassium are the major elements needed by salad crops and currently require resupply on Station. However, it is feasible that these macronutrients could be recovered through the waste materials generated by the crew. The proposed concepts are non-oxidative and simple in design. This paper considers the potential for reclaiming macronutrients from urine and gray water concentrates from water recovery systems.

This project is a Phase III SBIR contract between NASA and Water Reuse Technology (WRT). It covers the redesign, modification, and construction of the Wiped-Film Rotating-Disk (WFRD) evaporator for use in microgravity and its integration into a Vapor Phase Catalytic Ammonia Removal (VPCAR) system. VPCAR is a water processor technology for long duration space exploration applications. The system is designed as an engineering development unit specifically aimed at being integrated into NASA Johnson Space Center's Bioregenerative Planetary Life Support Test Complex (BIO-Plex). The WFRD evaporator and the compressor are being designed and built by WRT. The balance of the VPCAR system and the integrated package are being designed and built by Hamilton Sundstrand Space Systems International, Inc. (HSSSI) under a subcontract with WRT. This paper provides a description of the VPCAR technology and the advances that are being incorporated into the unit.

This paper covers the development of computer simulation models of the Vapor Compression Distillation (VCD) process, the Super Critical Water Oxidation (SCWO) process, and two versions of a Vapor Phase Catalytic Ammonia Reduction (VPCAR) process. These process level models have combined into two Integrated Water Reclamation Systems (IWRS). Results from these integrated models, in conjunction with other data sources, have been used to develop a preliminary comparison of the two systems. Also discussed in this paper is the development of a Vapor Phase Catalytic Ammonia Reduction teststand and the development of a new urine analog for use with the teststand and computer models.

The General Purpose Work Station (GPWS) is a laboratory multi-use facility, as demonstrated during the Spacelab Life Sciences 1 (SLS-1) flight. The unit provided particulate containment under varying conditions, served as an effective work space for manipulating live animals, e.g., rats, served as a containment facility for fixatives, and was proposed for use to conduct in-flight maintenance during connector pin repair. The cabinet has a front door large enough to allow installation of a full-size microscope in-flight and is outfitted with a side window to allow delivery of items into the cabinet without exposure to the spacelab atmosphere. Additional support subsystems include inside cabinet mounting, surgical glove fine manipulations capability, and alternating or direct current power supply for experiment equipment, as will be demonstrated during Spacelab J.

NASA is currently developing two new human rated launch systems. They are the Crew Exploration Vehicle (CEV) and the Lunar Surface Access Module (LSAM). Both of these spacecraft will require new life support systems to support the crew. These life support systems can also be designed to reduce the mass required to keep humans alive in space. Water accounts for about 80% of the mass required to keep a person alive. As a result recycling water offers a high return on investment. Recycling water can also increase mission safety by providing an emergency supply of drinking water. This paper evaluates the potential benefits of two wastewater treatment technologies that have been designed to reduce the mass of the CEV and LSAM missions. For a 3 day CEV mission to the International Space Station (ISS) this approach could reduce the mass required to provide drinking water by 65% when compared to stored water. For an 18 day Lunar mission a mass savings of 70% is possible.

The Vapor Phase Catalytic Ammonia Removal (VPCAR) system is being developed to recycle water for future NASA Exploration Missions. Testing was recently conducted on NASA's C-9B Reduced Gravity Aircraft to determine the microgravity performance of a key component of the VPCAR water recovery system. Six flights were conducted to evaluate the fluid dynamics of the Wiped-Film Rotating Disk (WFRD) distillation component of the VPCAR system in microgravity, focusing on the water delivery method. The experiments utilized a simplified system to study the process of forming a thin film on a disk similar to that in the evaporator section of VPCAR. Fluid issues are present with the current configuration, and the initial alternative configurations were only partial successful in microgravity operation. The underlying causes of these issues are understood, and new alternatives are being designed to rectify the problems.

The Spacelab Life Sciences-2 (SLS-2) mission provided scientists with the unique opportunity of obtaining inflight rodent tissue and blood samples during a 14-day mission flown in October, 1993. In order to successfully obtain these samples, Ames Research Center's Space Life Sciences Payloads Office has developed an innovative, modular approach to packaging the instruments used to obtain and preserve the inflight tissue and blood samples associated with the hematology experiments on SLS-2. The design approach organized the multitude of instruments into 12 different 5x6x1 inch kits which were each used to accomplish a particular experiment functional objective on any given day during the mission. The twelve basic kits included blood processing, isotope and erythropoietin injection, body mass measurement, and microscope slides.