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A seat vibration prediction technique using a substructure synthesis method was developed for use in ride comfort evaluations. The human body was modeled as a vibration transfer matrix using the mean apparent mass of human subjects, based on data measured in advance. Seat vibration characteristics were measured with rigid masses on the seat. The measured data and vibration transfer matrix of the human body were synthesized using a substructure synthesis method, to predict vibration of the seat cushion and backrest in an occupant-loaded condition without actually using human subjects. Results showed that seat vibration predicted with this method was very similar to, and more repeatable than, that obtained experimentally with human subjects.