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Global warming and climate change have led to a greater interest in the implementation of biofuels in internal combustion engines. In spark ignited engines, biofuels have been shown to improve efficiency and knock resistance while decreasing emissions of unburned hydrocarbons, carbon monoxide and particles. This study investigates the effect of biofuels on SI engine combustion through a graphical compilation of previously reported results. Experimental data from 88 articles were used to evaluate the trends of the addition of different biofuels in gasoline. Graphs illustrating engine performance, combustion phasing and emissions are presented in conjunction with data on the physiochemical properties of each biofuel component to understand the observed trends. Internal combustion engines have the ability to handle a wide variety of fuels resulting in a broad range of biofuel candidates.