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In this study, a fully optically accessible single-cylinder research engine is the basis for the visualization and generation of extensive knowledge about the in-cylinder processes of mixture formation, ignition and combustion of oxygenated synthetic fuels. Previous measurements in an all-metal engine showed promising results by using a mixture of dimethyl carbonate and methyl formate as a fuel substitute in a DISI-engine. Lower THC and NOx emissions were observed along with a low PN-value, implying low-soot combustion. The flame luminosity transmitted via an optical piston was split in the optical path to simultaneously record the natural flame luminosity with an RGB high-speed camera. The second channel consisted of OH*-chemiluminescence recording, isolated by a bandpass filter via an intensified monochrome high-speed camera.