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In this paper, the authors have developed a new measuring method of the liquid fuel film thickness on walls, such as intake ports, the combustion chamber and cylinder liner of a Port Fuel Injection (PFI) engine, and clarified the fuel film behavior under various running conditions when Fiber-based Laser-Induced Fluorescence (Fiber-based LIF) was applied to the newly developed method. The thickness of the fuel film is measured by detecting the intensity of fluorescence from the film that is irradiated by a He-Cd laser. A single optical fiber is used to simultaneously transmit the laser beam and the fluorescence from the film. In addition, the S/N ratio of the fluorescence is improved by using a He-Cd laser of which the wavelength (λ=442nm) is able to efficiently irradiate test fuel doped 2-3-butandione. Using this method, the fuel film thickness on the wall of the PFI engine was analyzed in two case studies.