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The burn rate and the instantaneous in-cylinder heat transfer have been studied experimentally in a spray-guided direct-injection spark-ignition engine with three different fuels: gasoline, iso-octane and toluene. The effects of the ignition timing, air fuel ratio, fuel injection timing and injection strategy (direct injection or port injection) on the burn rate and the in-cylinder heat transfer have been experimentally investigated at a standard mapping point (1500 rpm and 0.521 bar MAP) with the three different fuels. The burn rate analysis was deduced from the in-cylinder pressure measurement. A two-dimensional heat conduction model of the thermocouple was used to calculate the heat flux from the measured surface temperature. An engine thermodynamic simulation code was used to predict the gas-to-wall heat transfer.