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Biological wastewater processing has been under investigation by AlliedSignal Aerospace and NASA Johnson Space Center (JSC) for future use in space. Testing at JSC in the Hybrid Regenerative Water Recovery System (HRWRS) in preparation for future closed human testing has been performed. Computer models have been developed to aid in the design of a new four-person immobilized cell bioreactor. The design of the reactor and validation of the computer model is presented. In addition, the total organic carbon (TOC) computer model has been expanded to begin investigation of nitrification. This model is being developed to identify the key parameters of the nitrification process, and to improve the design and operating conditions of nitrifying bioreactors. In addition, the model can be used as a design tool to rapidly predict the effects of changes in operational conditions and reactor design, significantly reducing the number and duration of experiments required.

The NASA Johnson Space Center has plans to integrate a Solid Amine Water Desorbed (SAWD II) carbon dioxide removal subsystem into the Variable Pressure Growth Chamber (VPGC). The SAWD II subsystem will be used to remove any excess carbon dioxide (CO2) input into the VPGC which is not assimilated by the plants growing in the chamber. An analysis of the integrated VPGC-SAWD II system was performed using a mathematical model of the system implemented in the Computer-Aided System Engineering and Analysis (CASE/A) package. The analysis consisted of an evaluation of the SAWD II subsystem configuration within the VPGC, the planned operations for the subsystem, and the overall performance of the subsystem and other VPGC subsystems. Based on the model runs, recommendations were made concerning the SAWD II subsystem configuration and operations, and the chambers' automatic CO2 injection control subsystem.

The Early Human Testing Initiative (EHTI) Phase I Human Test, performed by the Crew and Thermal Systems Division at Johnson Space Center, demonstrated the ability of a crop of wheat to provide air revitalization for a human test subject for a 15-day period. The test demonstrated three different methods for control of oxygen and carbon dioxide concentrations for the human/plant system and obtained data on trace contaminants generated by both the human and plants during the test and their effects on each other. The crop was planted in the Variable Pressure Growth Chamber (VPGC) on July 24, 1995 and the test subject entered the adjoining airlock on day 17 of the wheat's growth cycle. The test subject stayed in the chamber for a total of 15 days, 1 hour and 20 minutes. Air was mixed between the plant chamber and airlock to provide oxygen to the test subject and carbon dioxide to the plants by an interchamber ventilation system.