Search Results

An integrated water recovery system was operated for 91 days in support of the Lunar Mars Life Support Test Project (LMLSTP) Phase III test. The system combined both biological and physical-chemical processes to treat a combined wastewater stream consisting of waste hygiene water, urine, and humidity condensate. Biological processes were used for primary degradation of organic material as well as for nitrification of ammonium in the wastewater. Physical-chemical systems removed inorganic salts from the water and provided post-treatment. The integrated system provided potable water to the crew throughout the test. This paper describes the water recovery system and reviews the performance of the system during the test.

Although bioprocessors have been successfully tested in ground test experiments as primary wastewater processors [1, 2 and 3], the transition required for operation of a bioprocessor in microgravity is complicated by the absence of gravity and buoyancy-driven convection. Gases are present in the wastewater bioprocessor from numerous sources including aeration, metabolic production and operation. This paper presents an innovative approach to the delivery of metabolically-required oxygen to a bioprocessor. A bioprocessor that provides oxygen delivery and bacterial support using membranes has been developed and tested during the past two years. Bench-top laboratory results have demonstrated that Total Organic Carbon (TOC) degradation above 95%, and nitrification above 80% can be maintained, while denitrification typically ranged between 5-25% in a membrane bioprocessor system (MBS).

In a crewed spacecraft environment, atmospheric carbon dioxide (CO2) and moisture control are crucial. Hamilton Sundstrand has developed a stable and efficient amine-based CO2 and water vapor sorbent, SA9T, that is well suited for use in a spacecraft environment. The sorbent is efficiently packaged in pressure-swing regenerable beds that are thermally linked to improve removal efficiency and minimize vehicle thermal loads. Flows are controlled with a single spool valve. This technology has been baselined for the new Orion spacecraft, but additional data was needed on the operational characteristics of the package in a simulated spacecraft environment. One unit was tested with simulated metabolic loads in a closed chamber at Johnson Space Center during the latter part of 2006. Those test results were reported in a 2007 ICES paper.

Thermal control systems for space application plant growth chambers offer unique challenges. The ability to control temperature and humidity independently gives greater flexibility for optimizing plant growth. Desired temperature and relative humidity range vary widely from 15°C to 35°C and 65% to 85% respectively. On top of all of these variables, the thermal control system must also be conservative in power and mass. These requirements to develop and test a robust thermal control system for space applications led to the design and development of the Environmental System Test Stand (ESTS) at NASA Johnson Space Center (JSC). The ESTS was designed to be a size constrained, environmental control system test stand with the flexibility to allow for a variety of thermal and lighting technologies. To give greater understanding to the environmental control system, the development of the ESTS included both mathematical models and the physical test stand.

Bioregenerative life support systems (BLSS) may be necessary for long-term space missions due to the high costs of lifting supplies and equipment into orbit. Much of the recycling to be done in a BLSS involves microbial activity. Although most studies to date have used a culture-based approach to characterize bacteria in BLSS under development, recently work has begun utilizing non-culture-based, DNA approaches to elucidate which microbes are present. In this study, we investigated whether replicate reactors develop replicate microbial communities using a 16S rRNA gene approach and terminal restriction length polymorphism analysis for tubular, nitrifier reactors in use at JSC. Our result suggests that both individual reactor and temporal signals can be detected in the microbial populations. This information may lead to optimization of inoculation procedures and reactor operations conditions to increase predictability and reliability of biological systems.

Biological water processors are currently being developed for application in microgravity environments. Work has been performed to develop a single-phase, gravity independent anoxic denitrification reactor for organic carbon removal [1]. As a follow on to this work it was necessary to develop a gravity independent nitrification reactor in order to provide sufficient nitrite and nitrate to the organic carbon oxidation reactor for the complete removal of organic carbon. One approach for providing the significant amounts of dissolved oxygen required for nitrification is to require the biological reactor design to process two-phase gas and liquid in micro-gravity. This paper addresses the design and test results overview for development of a tubular, two-phase, gravity independent nitrification biological water processor.

The work presented in this paper summarizes the early results of an integrated advanced water recovery system test conducted by the Crew and Thermal Systems Division (CTSD) at NASA-Johnson Space Center (JSC). The system design and the results of the first two months of operation are presented. The overall objective of this test is to demonstrate the capability of an integrated advanced water recovery system to produce potable quality water for at least six months. Each subsystem is designed for operation in microgravity. The primary treatment system consists of a biological system for organic carbon and ammonia removal. Dissolved solids are removed by reverse osmosis and air evaporation systems. Finally, ion exchange technology in combination with photolysis or photocatalysis is used for polishing of the effluent water stream. The wastewater stream consists of urine and urine flush water, hygiene wastewater and a simulated humidity condensate.

This paper provides an overview of the IMMWPS Integrated Ground Test Program, completed at the NASA Johnson Space Center (JSC) during October and November 2001. The JSC Crew and Thermal Systems Division (CTSD) has developed the IMMWPS orbital flight experiment to test the feasibility of a microbe-based water purifier for use in zero-gravity conditions. The IMMWPS design utilizes a Microbial Processor Assembly (MPA) inoculated with facultative anaerobes to convert organic contaminants in wastewater to carbon dioxide and biomass. The primary purpose of the ground test program was to verify functional operations and procedures. A secondary objective was to provide initial ground data for later comparison to on-orbit performance. This paper provides a description of the overall test program, including the test article hardware and the test sequence performed to simulate the anticipated space flight test program. In addition, a summary of significant results from the testing is provided.

The International Space Station Waste Collector Subsystem Risk Mitigation Experiment (ISS WCS RME) was flown as the primary (Shuttle) WCS on Space Shuttle flight STS-104 (ISS-7A) in July 2001, to validate new design enhancements. In general, the WCS is utilized for collecting, storing, and compacting fecal & associated personal hygiene waste, in a zero gravity environment. In addition, the WCS collects and transfers urine to the Shuttle waste storage tank. All functions are executed while controlling odors and providing crew comfort. The ISS WCS previously flew on three Shuttle flights as the Extended Duration Orbiter (EDO) WCS, as it was originally designed to support extended duration Space Shuttle flights up to 30 days in length. Soon after its third flight, the Space Shuttle Program decided to no longer require 30 day extended mission duration capability and provided the EDO WCS to the ISS Program.

Two crops of lettuce (Lactuca sativa cv. Waldmann's Green) were grown in the Regenerative Life Support Systems (RLSS) Test Bed at NASA's Johnson Space Center. The RLSS Test Bed is an atmospherically closed, controlled environment facility for the evaluation of regenerative life support systems using higher plants. The chamber encloses 10.6 m2 of growth area under cool-white fluorescent lamps. Lettuce was double seeded in 480 pots, each containing about 250 cm3 of calcined-clay substrate. Each pot was irrigated with half-strength Hoagland's nutrient solution at an average total applied amount of 2.5 and 1.8 liters pot-1, respectively, over each of the two 30-day crop tests. Average environmental and cultural conditions during both tests were 23°C air temperature, 72% relative humidity, 1000 ppm carbon dioxide (CO2), 16h light/8h dark photoperiod, and 356 μmol m-2s-1 photosynthetic photon flux.

Biological wastewater processing has been under investigation by AlliedSignal Aerospace and NASA Johnson Space Center (JSC) for future use in space. Testing at JSC in the Hybrid Regenerative Water Recovery System (HRWRS) in preparation for future closed human testing has been performed. Computer models have been developed to aid in the design of a new four-person immobilized cell bioreactor. The design of the reactor and validation of the computer model is presented. In addition, the total organic carbon (TOC) computer model has been expanded to begin investigation of nitrification. This model is being developed to identify the key parameters of the nitrification process, and to improve the design and operating conditions of nitrifying bioreactors. In addition, the model can be used as a design tool to rapidly predict the effects of changes in operational conditions and reactor design, significantly reducing the number and duration of experiments required.

Approximately 50% of the water consumed by International Space Station crewmembers is water recovered from cabin humidity condensate. Condensing heat exchangers in the Russian Service Module (SM) and the United States On-Orbit Segment (USOS) are used to control cabin humidity levels. In the SM, humidity condensate flows directly from the heat exchanger to a water recovery system. In the USOS, a metal bellows tank located in the US Laboratory Module (LAB) collects and stores condensate, which is periodically off-loaded in about 20-liter batches to Contingency Water Containers (CWCs). The CWCs can then be transferred to the SM and connected to a Condensate Feed Unit that pumps the condensate from the CWCs into the water recovery system for processing. Samples of the condensate in the tank are collected during the off-loads and returned to Earth for analyses.

For over 40 years, NASA has been putting humans safely into space in part by minimizing microbial risks to crew members. Success of the program to minimize such risks has resulted from a combination of engineering and design controls as well as active monitoring of the crew, food, water, hardware, and spacecraft interior. The evolution of engineering and design controls is exemplified by the implementation of HEPA filters for air treatment, antimicrobial surface materials, and the disinfection regimen currently used on board the International Space Station. Data from spaceflight missions confirm the effectiveness of current measures; however, fluctuations in microbial concentrations and trends in contamination events suggest the need for continued diligence in monitoring and evaluation as well as further improvements in engineering systems. The knowledge of microbial controls and monitoring from assessments of past missions will be critical in driving the design of future spacecraft.

The Shuttle Orbiter humidity control and carbon dioxide removal system for extended duration missions presently uses a solid amine called HS-C. This August, on board STS-62, a new solid amine called HS-C+ will be used. HS-C+ uses the same amine and the substrate material, but a different preparation process. Forty-seven breakthrough tests have been conducted to characterize the performance of HS-C+. CO2 partial pressure, bed temperature, and H2O partial pressure were varied. Eleven HS-C breakthrough tests were also run to provide a direct comparison. Under all conditions tested, HS-C+ outperformed HS-C. Both materials adsorb all CO2 and H2O available at the start of a test when the beds are fully desorbed. As the bed becomes partially loaded, the CO2 and H2O adsorption rates decrease rapidly. HS-C+ continues adsorbing all CO2 and H2O available for a longer time. Greater surface area on HS-C+ may cause the improved performance.

A primary concern in creating a water reclamation system for long-duration manned space flight is the control of microbial contamination which can jeopardize water quality, compromise human health, and degrade constituent materials of the system. The microbial ecology facility in the Analytical and Physical Chemistry Branch of the Materials and Processes Laboratory at NASA's Marshall Space Flight Center (MSFC) is addressing this concern by means of experiments investigating the interaction of bacterial species in the development of a biofilm and their response to the introduction of additional species or to disinfection. Both static and recycling water systems are used. In static experiments, varied sequence and timing of species introduction in binary bacterial biofilms on 316L stainless steel elucidate the mechanisms involved in biofilm formation.

Two simulated spacecraft water systems are being used to evaluate the effectiveness of iodine for controlling microbial contamination within such systems. An iodine concentration of about 2.0 mg/L is maintained in one system by passing ultrapure water through an iodinated ion exchange resin. Stainless steel coupons with electropolished and mechanically-polished sides are being used to monitor biofilm formation. Results after three years of operation show a single episode of significant bacterial growth in the iodinated system when the iodine level dropped to 1.9 mg/L. This growth was apparently controlled by replacing the iodinated ion exchange resin, thereby increasing the iodine level. The second batch of resin has remained effective in controlling microbial growth down to an iodine level of 1.0 mg/L. Scanning electron microscopy indicates that the iodine has impeded but may have not completely eliminated the formation of biofilm.

The Space Shuttle humidity separator prefilter was developed to remove debris from the air/water stream that flows from the cabin condensing heat exchanger to the humidity separator. Debris in this flow stream has caused humidity separator pitot tube clogging and subsequent water carryover on several Shuttle flights. The first design concept of the prefilter was flown on STS-40 in June, 1991. The prefilter was installed on-orbit. Video footage of its operation revealed that the prefilter did not pass water at a constant rate, resulting in a tendency to slug the humidity separator. The results from this flight test have resulted in a complete redesign of the prefilter. In this paper the first prefilter design is described, the flight results from STS-40 are examined, and the on-orbit performance of the prefilter is explained. The redesigned prefilter is described with emphasis on the features that should allow successful reduced gravity operation.

Life Sciences research on Space Station will utilize rats to study the effects of the microgravity environment on mammalian physiology and to develop countermeasures to those effects for the health and safety of the crew. The animals will produce metabolic water which must be reclaimed to minimize logistics support. The condensate from the Research Animal Holding Facility (RAHF) flown on Spacelab Life Sciences-2 (SLS-2) in October 1993 was used as an analog to determine the type and quantity of constituents which the Space Station (SS) water reclamation system will have to process. The most significant organics present in the condensate were 2-propanol, glycerol, ethylene glycol, 1,2-propanediol, acetic acid, acetone, total proteins, urea and caprolactam while the most significant inorganic was ammonia. Microbial isolates included Xanthomonas, Sphingobacterium, Pseudomonas, Penicillium, Aspergillus and Chrysosporium.

Controlling microbial growth and biofilm formation in spacecraft water-distribution systems is necessary to protect the health of the crew. Methods to decontaminate the water system in flight may be needed to support long-term missions. We evaluated the ability of iodine and ozone to kill attached bacteria and remove biofilms formed on stainless steel coupons. The biofilms were developed by placing the coupons in a manifold attached to the effluent line of a simulated spacecraft water-distribution system. After biofilms were established, the coupons were removed and placed in a treatment manifold in a separate water treatment system where they were exposed to the chemical treatments for various periods. Disinfection efficiency over time was measured by counting the bacteria that could be recovered from the coupons using a sonication and plate count technique. Scanning electron microscopy was also used to determine whether the treatments actually removed the biofilm.

Integrating physicochemical and biological technologies into a regenerative life support system is a complex technical challenge. NASA recognizes that the depth and breadth of the challenge warrants a comprehensive investigation. NASA is implementing several ground-based projects to look at different systems integration issues. The combined efforts of these activities will enable NASA to develop regenerative life support systems for human exploration of the solar system in the 21st century. This paper provides an overview of NASA's overall approach to ground testing of integrated regenerative life support systems.