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Alternative fuels are being evaluated in automotive applications in both commercial and government fleets in an effort to reduce emissions and United States dependence on diesel fuel. Vehicles and equipment have been operated using 100 percent biodiesel and various blends of biodiesel and diesel fuel in a variety of applications, including farming equipment and transit buses. This government study investigates the compatibility of four base fuels and six blends with elastomer and metallic components commonly found in fuel systems. The physical properties of the elastomers were measured according to American Society of Testing and Materials (ASTM) D 471, “Standard Test Method for Rubber Property-Effect of Liquids,” and ASTM D 412, “Standard Test Methods for Rubber Properties in Tension.” These evaluations were performed at 51.7°C for 0, 22, 70, and 694 hours. Tensile strength, hardness, swell, and elongation were determined for all specimens.