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Particle Image Velocimetry (PIV) technique was used to study the in-cylinder flow motion in a single cylinder transparent spark ignition (SI) engine, with 4 valves pent-roof cylinder-head. Experiments have been firstly performed using classical PIV technique in the symmetrical plane of the combustion chamber. The instantaneous two-dimensional velocity fields show that during intake stroke, the flow has strong relatively stable structures with low cycle by cycle (CBC) variations, whereas during compression stroke, the flow consists of large unstable eddies with high CBC variations. In all cases, no large tumble-like motion was observed on instantaneous fields in the measurement plane during the intake stroke. Therefore, Stereoscopic Particle Image Velocimetry (SPIV) technique was adapted to complete the classical two-dimensional measurements and in order to have a better understanding of the in-cylinder flow during compression stroke.