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Experiments were conducted with a commercially available six-cylinder, 4-valves per cylinder, turbocharged, direct injection (DI) diesel engine. The engine was operated with low sulfur diesel fuel, ultra low sulfur diesel fuel and two other blends, low sulfur diesel fuel with 20 wt.% biodiesel and ultra low sulfur diesel fuel with 20 wt.% biodiesel, to investigate the effect of the base fuels and their blends on combustion and emissions. Combustion analysis, particulate matter emissions and exhaust gas composition (CO, NOX and total hydrocarbons) were determined at eight steady-state operating conditions according to the AVL 8-Mode test protocol. Combustion analysis showed at high load conditions a retarded start of injection, an earlier start of combustion and a lower premixed burn peak with ultra low sulfur diesel fuel. Mode averaged NOX emissions decreased with ultra low sulfur diesel fuel and biodiesel blends compared to low sulfur diesel fuel.