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Renewable fuels have an important role to create sustainable energy systems. In this paper the focus is on biodiesel, which is produced from vegetable oils or animal fats. Today biodiesel is mostly used as a drop-in fuel, mixed into conventional diesel fuels to reduce their environmental impact. Low quality drop-in fuel can lead to deposits throughout the fuel systems of heavy duty vehicles. In a previous study fuel filters from the field were collected and analyzed with the objective to determine the main components responsible for fuel filter plugging. The identified compounds were constituents of soft particles. In the current study, the focus was on metal carboxylates since these have been found to be one of the components of the soft particles and associated with other engine malfunctions as well. Hence the measurement of metal carboxylates in the fuel is important for future studies regarding the fuel’s effect on engines.