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Biodiesel as a renewable energy is becoming increasingly attractive due to the growing scarcity of conventional fossil fuels. Meanwhile, the development of after-treatment technologies for the diesel engine brings new insight concerning emissions especially the particulate matter pollutants. In order to study the coupling effects of biodiesel blend and CCRT (Catalyzed Continuously Regeneration Trap) on the particulate matter emissions, the particulate matter emissions from an urban bus with and without CCRT burning BD0 and BD10 respectively was tested and analyzed using electrical low pressure impactor (ELPI). The operation conditions included steady state conditions and transient conditions. Results showed that the particulate number-size distribution of BD10 and BD0 both had two peaks in nuclei mode and accumulation mode at the conditions of idle, low speed and medium speed while at high speed condition the particulate number-size distribution only had one peak.