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Biofuels, such as ethanol and butanol, have been the subject of significant political and scientific attention, owing to concerns about climate change, global energy security, and the decline of world oil resources that is aggravated by the continuous increase in the demand for fossil fuels. This study evaluated the potential emissions impacts of different alcohol blends on a fleet of modern gasoline vehicles. Testing was conducted on a fleet of nine vehicles with different combinations of ten fuel blends over the Federal Test Procedure and Unified Cycle. The vehicles ranged in model year from 2007-2014 and included four vehicles with port fuel injection (PFI) fueling and five vehicles with direct injection (DI) fueling. The ten fuel blends included ethanol blends at concentrations of 10%, 15%, 20%, 51%, and 83% by volume and iso-butanol blends at concentrations of 16%, 24%, 32%, and 55% by volume, and an alcohol mixture giving 10% ethanol and 8% iso-butanol in the final blend.