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Nitrogen oxide (NOx) sensing is required both for on-board diagnosis and optimal selective catalytic reduction (SCR)-catalyst control in heavy duty diesel engines. This can be accomplished either by physical solid-state sensors, or by so-called virtual sensors, which estimate the value of the target quantity using other states by means of a model. Both approaches have advantages and disadvantages. This paper resumes the derivation and the identification of a virtual sensor based on a polynomial structure and optimal experimental design methods and compares its performance to the one of a production physical solid state sensor. The virtual sensor is compared with a commercially available solid-state sensor in terms of accuracy (stationary as well as dynamic) and operation limits.