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Ambulances have different performance needs and structural design compared to standard passenger vehicles. Also occupants in the ambulance rear patient compartment maybe side facing, rear facing or recumbent. There is also no USA dynamic safety standard for testing the ambulance patient compartment occupant or equipment restraint systems. This study describes an accelerator sled test conducted of an ambulance rear cabin environment which demonstrates some optimal restraint practices for pediatric patients and also the interaction between the different occupants and the need for effective restraint systems. The goal of this study was to analyze occupant kinematics and forces generated in a model of an ambulance crash, and to test injury-mitigating countermeasures for both pediatric and adult occupants.