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In an optically-accessible single-cylinder engine fuelled with hydrogen, OH* chemiluminescence imaging and planar laser induced fluorescence (PLIF) are used to qualitatively evaluate in-cylinder mixture formation. The experiments include measurements for engine operation with hydrogen injection in-cylinder either prior to or after intake valve closure (IVC). Pre-IVC injection is used to produce a near homogeneous mixture in-cylinder to establish a baseline comparison for post-IVC injection. To assess the effects of injection pressure on mixture formation, two injection pressures are used for post-IVC injection. For post-IVC injection with start of injection (SOI) coincident with IVC, mixture distribution is similar to pre-IVC injection and there are little differences between the two injection pressures. With retard of SOI from IVC, mixture inhomogeneities increase monotonically for both injection pressures.