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Ignition quality tester (IQT) is a standard experimental device to determine ignition delay time of liquid fuels in a controlled environment in the absence of gas exchange. The process involves fuel injection, spray breakup, evaporation and mixing, which is followed by auto-ignition. In this study, three-dimensional computational fluid dynamics (CFD) is used for prediction of auto-ignition characteristics of diethyl ether (DEE) and ethanol. In particular, the sensitivity of the ignition behavior to different injection rate profiles is investigated. Fluctuant rate profile derived from needle lift data from experiments performs better than square rate profile in ignition delay predictions. DEE, when used with fluctuant injection rate profile resulted in faster ignition, while for ethanol the situation was reversed. The contrasting results are attributed to the difference in local mixing.