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Visual sensors are widely used in autonomous vehicles (AVs) for object detection due to the advantages of abundant information and low-cost. But the performance of visual sensors is highly affected by low light conditions when AVs driving at nighttime and in the tunnel. The low light conditions decrease the image quality and the performance of object detection, and may cause safety of the intended functionality (SOTIF) problems. Therefore, to analyze the performance limitations of visual sensors in low light conditions, a controlled light experiment on a proving ground is designed. The influences of low light conditions on the two-stage algorithm and the single-stage algorithm are compared and analyzed quantificationally by constructing an evaluation index set from three aspects of missing detection, classification, and positioning accuracy.