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Gas diffusion layer (GDL), as a critical constituent of the proton exchange membrane fuel cell (PEMFC), plays a key role in mass, heat, electron, and species transport. GDL generally has two distinct layers: a macro-porous substrate (MPS) and a micro-porous layer (MPL). The fibers in MPS and the cracks formed during the deposition process on the surface of MPL change the overall transport capacity and effect the output performance of PEMFC. In this paper, methods of identifying the structural features of fibers and cracks in GDL images based on artificial intelligence are proposed. The block probabilistic Hough transform and the quadric voting based on the weighted K-means algorithm are programmed to realize the fiber feature extraction, and the crack feature extraction is realized by the regional connectivity algorithm and the geometric feature calculation based on the circumscribed graph of the crack region.