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In recent years, various biodiesels have been developed to decrease pollutant emissions from compression ignition engine. However, the current research focuses on reducing the pollutant components without considering the mechanical vibration that occurred due to the changes in fuel properties such as viscosity, calorific values, density, and bulk modulus. It is important to explore the relationships between fuel properties and engine vibration. Mechanical vibration could cause power loss and affect the lifetime of the engine. In this investigation, a lister-pitter 3-cylinder diesel engine was used to analyse the mechanical vibration of three different fuels including diesel, waste cooking oil biodiesel (WCOB), and lamb fat biodiesel (LFB). The high-frequency vibration sensors were mounted on the cylinder head to monitor and assess the vibration performance.