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The scattering properties (influenced by morphology) and refractive index (dependent on microstructure) of engine-emitted soot influences its effect on climate, as well as how we interpret optical measurements of aerosols. The morphology and microstructure of soot from two different engines were studied. The soot samples were collected from a 1.9L Volkswagen TDI engine for two different fuel types (ULSD and B20) and six speed/load combinations., as well as from a Cummins ISX heavy-duty engine using the Westport pilot-ignited high-pressure direct-injection (HPDI) natural-gas fuelling system for three different speed/load combinations. The transmission electron microscopy (TEM) was employed to investigate the soot morphology, emphasizing the fractal properties. Image processing was used to extract the geometrical properties of the thirty-five randomly chosen aggregates from each sample.