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In this study a mixture of dimethyl carbonate (DMC) and methyl formate (MeFo) was used as a synthetic gasoline replacement. These synthetic fuels offer CO2-neutral mobility if the fuels are produced in a closed CO2-cycle and they reduce harmful emissions like particulates and NOX. For base potential investigations, a single-cylinder research engine (SCE) was used. An in-depth analysis of real driving cycles in a series 4-cylinder engine (4CE) confirmed the high potential for emission reduction as well as efficiency benefits. Beside the benefit of lower exhaust emissions, especially NOX and particle number (PN) emissions, some additional potential was observed in the SCE. During a start of injection (SOI) variation it could be detected that a late SOI of DMC/MeFo has less influence on combustion stability and ignitability. With this widened range for the SOI the engine application can be improved for example by catalyst heating or stratified mode.