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Some fuels with the same research octane number (RON) have different HCCI engine performance. Therefore RON alone cannot be used for determining auto-ignition in HCCI combustion. The current research focuses on creating an HCCI fuel index suitable for comparing different fuels for HCCI operation. More thorough studies are needed to map the fuel effects. One way to characterize a fuel is by using the Auto-Ignition Temperature (AIT). The AIT and the amount of Low Temperature Heat Release (LTHR) together describe the auto-ignition properties of the fuel. Both can be extracted from the pressure trace. The assumption is that the pressure and temperature are known at inlet valve closing (IVC) and that the mass in the cylinder does not change after IVC. The purpose of this study was to map the AIT of different Primary Reference Fuels (PRF) for HCCI combustion at different cylinder pressures.