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In the Closed Ecology Experiment Facilities (CEEF), with integrating the Closed Plantation Experiment Facilities (CPEF) and the Closed Animal Breading & Habitation Facilities (CABHF), closed habitation experiments without material exchange with the outside will be conducted after the 2005 fiscal year. Cultivation experiments of about 30 crops and the integrating test of the material circulation system required for the closed habitation experiments have been performed since 2000 fiscal year. Using data reported in these experiments, material circulation in CEEF is simulated based on the recent operation strategy, and the storage capacity needed for the buffer of an air processing subsystem was estimated. In order for two humans to dwell over 120 days, the storage capacities of the carbon dioxide tank, the oxygen tank, and the waste gas tank in CPEF, and the carbon dioxide tank and the oxygen tank in CABHF are 820 g, 2830 g, 4425 g, 1780 g, and 1792 g, respectively.