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The Microbial Check Valve (MCV) is a canister containing an iodinated ion exchange resin and is used on the Shuttle Orbiter to provide microbial control of potable water. The MCV provides a significant contact kill, and imparts a biocidal iodine residual to the water. The Orbiter MCV has a design life of 30 days. For longer duration applications, such as Space Station Freedom, an extended life is desirable to avoid resupply penalties. A method of in situ MCV regeneration with elemental iodine is being developed. During regeneration water en route to the MCV first passes through a crystalline iodine bed where a concentration between 200 - 300 mg/L I2 is attained. When introduced into the MCV, this high concentration causes an equilibrium shift towards iodine loading, effecting regeneration of the resin. After regeneration normal flow is re-established. Life cycle regeneration testing is currently in progress.

The Microbial Check Valve (MCV) is used on the Space Shuttle to impart an iodine residual to the drinking water to maintain microbial control. Approximately twenty MCV locations have been identified in the Space Station Freedom design, each with a 90 day life. This translates to 2400 replacement units in 30 years of operation. An in situ regeneration concept has been demonstrated that will reduce this replacement requirement to less than 300 units based on data to date and potentially fewer as further regenerations are accomplished. A totally automated system will result in significant savings in crew time, resupply requirements and replacement costs. An additional feature of the device is the ability to provide a concentrated biocide source (200 mg/liter of I2) that can be used to superiodinate systems routinely or after a microbial upset. This program was accomplished under NASA Contract Number NAS9-18113.

On the International Space Station (ISS), humidity condensate will be collected from the atmosphere and treated by multifiltration to produce potable water for use by the crews. Ground-based development tests have demonstrated that multifiltration beds filled with a series of ion-exchange resins and activated carbons can remove many inorganic and organic contaminants effectively from wastewaters. As a precursor to the use of this technology on the ISS, a demonstration of multifiltration treatment under microgravity conditions was undertaken. On the Space Shuttle, humidity condensate from cabin air is recovered in the atmosphere revitalization system, then stored and periodically vented to space vacuum. A Shuttle Condensate Adsorption Device (SCAD) containing sorbent materials similar to those planned for use on the ISS was developed and flown on STS-68 as a continuation of DSO 317, which was flown initially on STS-45 and STS-47.

Controlling microbial growth and biofilm formation in spacecraft water-distribution systems is necessary to protect the health of the crew. Methods to decontaminate the water system in flight may be needed to support long-term missions. We evaluated the ability of iodine and ozone to kill attached bacteria and remove biofilms formed on stainless steel coupons. The biofilms were developed by placing the coupons in a manifold attached to the effluent line of a simulated spacecraft water-distribution system. After biofilms were established, the coupons were removed and placed in a treatment manifold in a separate water treatment system where they were exposed to the chemical treatments for various periods. Disinfection efficiency over time was measured by counting the bacteria that could be recovered from the coupons using a sonication and plate count technique. Scanning electron microscopy was also used to determine whether the treatments actually removed the biofilm.

The ability to aseptically remove samples and products, and the capability for addition of materials to sterile or otherwise microbially susceptible systems have always been compromised by the lack of a reliable means of sterilizing the mating fixtures. Cultures of mammalian cells are particularly vulnerable to microbial contamination due to the complexity of nutrient media and the lengthy periods required for cell growth. The Microwave Sterilizable Access Port has been developed to overcome this limitation. The system consists of three primary components: a microwave power source, a combined sterilization chamber/in-line valve port assembly, and a specimen transfer interface. Microwave energy is transmitted via coaxial cable to a small pressurized chamber that serves as a sterile transition between the surrounding environment and the system during transfer of materials.

Potable- and hygiene-quality water will be supplied to crews on the International Space Station through the recovery and purification of spacecraft wastewaters, including humidity condensate, urine, and wash water. Contaminants released into the cabin air from human metabolism, hardware offgassing, flight experiments, and routine operations will be present in spacecraft humidity condensate; normal constituents of urine and bathing water will be present in urine and untreated wash water. This report describes results from detailed analyses of Mir reclaimed potable water, ground-supplied water, and humidity condensate. These results are being used to develop and test water recycling and monitoring systems for the International Space Station (ISS); to evaluate the efficiency of the Mir water processors; and to determine the potability of the recycled water on board.

A significant decline in molecular iodine concentration is associated with the iodination of heavily contaminant-laden process water streams such as humidity condensate and urine distillate. Iodine loss is attributable to the reaction of this biocide with organic constituents. This phenomenon has been investigated using time resolved molecular absorption spectrophotometry of iodinated ersatz humidity condensates and iodinated ersatz urine distillates across the ultraviolet and visible spectral regions. Rates of iodine loss have also been studied using single contaminant systems at equivalent concentrations. The predominant reactive species have been identified as thiourea and formic acid. Pseudo-first order rate constants have been determined for ersatz contaminant model mixtures and for individual reactive constituents. Second order rate constants have been determined for the bimolecular reaction of iodine and formic acid.

Twenty-nine recycled water, eight stored (ground-supplied) water, and twenty-eight humidity condensate samples were collected on board the Mir Space Station during the Phase One Program (1995-1998). These samples were analyzed to determine potability of the recycled and ground-supplied water, to support the development of water quality monitoring procedures and standards, and to assist in the development of water reclamation hardware. This paper describes and summarizes the results of these analyses and lists the lessons learned from this project. Results show that the recycled water and stored water on board Mir, in general, met NASA, Russian Space Agency (RSA), and U.S. Environmental Protection Agency (EPA) standards.

Humidity condensate collected and processed in-flight is an important component of a space station drinking water supply. Water recovery systems in general are designed to handle finite concentrations of specific chemical components. Previous analyses of condensate derived from spacecraft and ground sources showed considerable variation in composition. Consequently, an investigation was conducted to collect condensate on the Shuttle while the vehicle was docked to Mir, and return the condensate to Earth for testing. This scenario emulates an early ISS configuration during a Shuttle docking, because the atmospheres intermix during docking and the condensate composition should reflect that. During the STS-89 and STS-91 flights, a total volume of 50 liters of condensate was collected and returned. Inorganic and organic chemical analyses were performed on aliquots of the fluid.

Two simulated spacecraft water systems are being used to evaluate the effectiveness of iodine for controlling microbial contamination within such systems. An iodine concentration of about 2.0 mg/L is maintained in one system by passing ultrapure water through an iodinated ion exchange resin. Stainless steel coupons with electropolished and mechanically-polished sides are being used to monitor biofilm formation. Results after three years of operation show a single episode of significant bacterial growth in the iodinated system when the iodine level dropped to 1.9 mg/L. This growth was apparently controlled by replacing the iodinated ion exchange resin, thereby increasing the iodine level. The second batch of resin has remained effective in controlling microbial growth down to an iodine level of 1.0 mg/L. Scanning electron microscopy indicates that the iodine has impeded but may have not completely eliminated the formation of biofilm.

The Microbial Check Valve (MCV) is a reloadable flow-through canister containing iodinated ion exchange resin, which is used aboard the Shuttle Orbiter as a disinfectant to maintain water potability. The MCV exhibits a significant contact kill and imparts a biocidal residual I2 concentration to the effluent. MCVs in current use have nominal 30 day lives. MCVs baselined for Space Station Freedom will have 90 day lives, and will require replacement 120 times over 30 years. Means to extend MCV life are desirable to minimize resupply penalties. New technology has been developed for fully autonomous in situ regeneration of an expended MCV canister. The Regenerative Microbial Check Valve (RMCV) consists of an MCV, a packed bed of crystalline I2, a flow diverter valve, an in-line iodine monitor and a microcontroller. During regeneration, flow is directed first through the packed I2 bed and then into the MCV where the resin is replenished.

A computer interfaced instrument designed to quantify ammonia and other nitrogenous species that are important regenerative life support water quality parameters is described in this paper. Measurements can be made either discretely by Flow Injection Analysis (FIA) or continuously using a split stream from a process flow. The monitor exhibits a predictable quadratic response for ammonia injections between 10 μg/L to 20 mg/L with a response time less than six minutes. A full description of the ammonia monitor's response to a variety of challenge solutions including real sewage treatment plant effluent is given. In addition, the response time characteristics, the effects of temperature, and interferences are described.

A Total Organic Carbon (TOC) Analyzer has been developed for a Life Sciences Risk Mitigation Flight Experiment to be conducted on Spacehab and the Russian space station, Mir. Initial launch is scheduled for December 1996 (flight STS-81). The analyzer will be tested on the Orbiter in the Spacehab module, including when the Orbiter is docked at the Mir space station. The analyzer is scheduled to be launched again in May 1997 (STS-84) when it will be transferred to Mir. During both flights the analyzer will measure the quality of recycled and ground-supplied potable water on the space station. Samples will be archived for later return to the ground, where they will be analyzed for comparison to in-flight results. Water test samples of known composition, brought up with the analyzer, also will be used to test its performance in microgravity. Ground-based analyses of duplicates of those test samples will be conducted concurrently with the in-flight analyses.