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A number of studies have been carried out examining the impact of biodiesel blend ratio on vehicle performance and emissions, however there is relatively little data available on the interaction between blend ratio and reduced ambient temperatures over the New European Drive Cycle (NEDC). This study examines the effects of increasing the blend ratio of Rapeseed Methyl Ester (RME) on the NEDC fuel consumption and tailpipe emissions of a vehicle equipped with a 2.0 litre common rail diesel engine, tested on a chassis dynamometer at ambient temperatures of 25, 10 & −5°C. This study found that under low temperature ambient conditions increasing blend ratios had a significant detrimental effect on vehicle particulate emissions reversing the benefits observed at higher ambient temperatures. Blend ratio was found to have minimal impact on hydrocarbon emissions regardless of ambient temperature while carbon monoxide and NOx emissions were found to increase by up to 20% and 5.5% respectively.