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There are many sources of air pollution that can threaten air quality during space missions. The International Space Station (ISS) is an extremely complex platform that depends on a multi-tiered strategy to control the risk of excessive air pollution. During the seven missions surveyed by this report, the ISS atmosphere was in a safe, steady-state condition; however, there were minor loads added as new modules were attached. There was a series of leaks of octafluoropropane, which is not directly toxic to humans, but did cause changes in air purification operations that disrupted the steady state condition. In addition, off-nominal regeneration of metal oxide canisters used during extravehicular activity caused a serious pollution incident.

Orbital Technologies Corporation (ORBITEC) and the University of Wisconsin (UW) have demonstrated the feasibility of utilizing plasma manufacturing methods to functionalize fluid handling surfaces. Performance of hydrophilic coatings generated with both oxygen plasma and dichlorosilane plasma on aluminum (SiH2Cl2) substrates was demonstrated. Both give similar results, significantly decreasing contact angles and improving wicking ability of machined capillary grooves. Deposition of silver nanoparticles using plasma was also demonstrated and tested. Silver concentrations of 2% were obtained on hydrophilic-coated samples. Testing indicated that the silver-coated samples were biocidal against Listeria monocytogenes. Oxide-coated aluminum substrates were also shown to exhibit biocidal action against L. monocytogenes and a variety of other microorganisms.

For over 40 years, NASA has been putting humans safely into space in part by minimizing microbial risks to crew members. Success of the program to minimize such risks has resulted from a combination of engineering and design controls as well as active monitoring of the crew, food, water, hardware, and spacecraft interior. The evolution of engineering and design controls is exemplified by the implementation of HEPA filters for air treatment, antimicrobial surface materials, and the disinfection regimen currently used on board the International Space Station. Data from spaceflight missions confirm the effectiveness of current measures; however, fluctuations in microbial concentrations and trends in contamination events suggest the need for continued diligence in monitoring and evaluation as well as further improvements in engineering systems. The knowledge of microbial controls and monitoring from assessments of past missions will be critical in driving the design of future spacecraft.

As part of its integrated system test bed capability, NASA's Advanced Life Support Program has undertaken the development of a large-scale advanced life support facility capable of supporting long-duration testing of integrated, regenerative biological and physicochemical life support systems. This facility--the Advanced Life Support Human-Rated Test Facility (HRTF) is currently being built at the Johnson Space Center. The HRTF is comprised of a series of interconnected chambers with a sealed internal environment capable of supporting a test crew of four for periods exceeding one year. The life support system will consist of both biological and physicochemical components and will perform air revitalization, water recovery, food production, solid waste processing, thermal management, and integrated command and control functions. Currently, a portion of this multichamber facility has been constructed and is being outfitted with basic utilities and infrastructure.

The 2001 Mars Odyssey spacecraft Martian Radiation Environment Experiment (MARIE) is a solid-state silicon telescope high-energy particle detector designed to measure galactic cosmic radiation (GCR) and solar particle events (SPEs) in the 20 – 500 MeV/nucleon energy range. In this paper we discuss the instrument design and focus on the observations and measurements of SPEs at Mars. These are the first-ever SPE measurements at Mars. The measurements are compared with the geostationary GOES satellite SPE measurements. We also discuss some of the current interplanetary particle propagation and diffusion theories and models. The MARIE SPE measurements are compared with these existing models.

The Advanced Animal Habitat (AAH) and Plant Research Unit (PRU) are two major components of the Space Station Biological Research Project (SSBRP). These two habitats are currently under development by Orbital Technologies Corporation (ORBITEC). Science Evaluation Units (SEUs) have been developed for each of these habitats to allow investigators to plan and test flight experiments on the ground using hardware that is functionally similar to the flight versions of the AAH and PRU. The SEUs also contain key functionality that makes them excellent science tools for general laboratory experiments that are not related to flight experiments.

Poly (vinyl chloride) (PVC) matrix membranes which incorporate the ionophore nonactin have been evaluated as cation exchange membranes for ammonium ion transport in an electrolytic cell configuration. Interest exists for the development of cation selective membranes for removal of low levels (<200ppm) of ammonium ions commonly found in recycled effluent streams in such diverse applications as expected in a Space Station and commercial fisheries. Ammonium ions are generated as a decomposition product of urea and over time build up in concentration, thus rendering the water unsuitable for human consumption. Nonactin is commonly used in a PVC matrix for ion-selective electrodes.

Biological wastewater processing has been under investigation by AlliedSignal Aerospace and NASA Johnson Space Center (JSC) for future use in space. Testing at JSC in the Hybrid Regenerative Water Recovery System (HRWRS) in preparation for future closed human testing has been performed. Computer models have been developed to aid in the design of a new four-person immobilized cell bioreactor. The design of the reactor and validation of the computer model is presented. In addition, the total organic carbon (TOC) computer model has been expanded to begin investigation of nitrification. This model is being developed to identify the key parameters of the nitrification process, and to improve the design and operating conditions of nitrifying bioreactors. In addition, the model can be used as a design tool to rapidly predict the effects of changes in operational conditions and reactor design, significantly reducing the number and duration of experiments required.

The Advanced Animal Habitat (AAH) represents the next generation of Space Station based animal research facilities. Care has been taken to protect the ISS crew from exposure to the hazardous biological materials contained within the AAH. These materials include rat feces, urine, dander, and odor. The approach to containing biological materials relies on collecting the solid and liquid waste, providing physical barriers between the waste and the crew environment, maintaining negative pressure within the specimen environment with respect to the crew environment, and providing odor filtration of air exchanged between the specimen and crew environments. These protections will be in place during all modes of AAH operation.

NASA's Advanced Life Support Program has included as part of its long-range planning the development of a large-scale advanced life support facility capable of supporting long-duration testing of integrated, regenerative biological and physicochemical life support systems. As the designated NASA Field Center responsible for integration and testing of advanced life support systems, Johnson Space Center has undertaken the development of such a facility--the Advanced Life Support Human-Rated Test Facility (HRTF). As conceived, the HRTF is an interconnected five-chamber facility with a sealed internal environment capable of supporting a test crew of four for periods exceeding one year. The life support system which sustains the crew consists of both biological and physicochemical components and will perform air revitalization, water recovery, food production, solid waste processing, thermal management, and integrated control and monitoring functions.

As part of the PRU project a new plant lighting system has been developed. System design focused on light source development, chamber optical performance improvements and electronics optimization. Central to the lighting system performance is a high density LED Light Engine, enabling increased spectral diversity, higher irradiance levels, enhanced uniformity and improved efficiency. Chamber wall surface materials were tested to minimize the vertical irradiance gradient and improve planar uniformity. Total lighting system efficiency was improved through the use of switching converter LED drive circuitry. As an alternative to the LED light source, an advanced planar fluorescent lighting source has also been developed.

We have conducted experiments on 16 lunar soils and 3 lunar volcanic glass samples to study the extraction of oxygen, an important resource for future lunar bases. The samples were chosen to span the range of composition and mineralogy represented in the Apollo collection. Each sample was reduced in flowing hydrogen for 3 hours at 1050°C. The dominant effect was reduction of Fe2+ (as FeO) in minerals and glass to iron metal, with concomitant release of oxygen. Oxygen extraction was strongly correlated with initial Fe2+ abundance but varied among mineral and glass phases. The experimental reduction of lunar soil and glass provides a method for assessing the oxygen production potential for sites on the lunar surface from lunar orbit. Our results show that oxygen yield from lunar soils can be predicted from knowledge of only one parameter, total iron content. This parameter can be measured from orbit by gamma ray spectrometry or multispectral imaging.

Integrating physicochemical and biological technologies into a regenerative life support system is a complex technical challenge. NASA recognizes that the depth and breadth of the challenge warrants a comprehensive investigation. NASA is implementing several ground-based projects to look at different systems integration issues. The combined efforts of these activities will enable NASA to develop regenerative life support systems for human exploration of the solar system in the 21st century. This paper provides an overview of NASA's overall approach to ground testing of integrated regenerative life support systems.

An integrated water recovery system was operated for 91 days in support of the Lunar Mars Life Support Test Project (LMLSTP) Phase III test. The system combined both biological and physical-chemical processes to treat a combined wastewater stream consisting of waste hygiene water, urine, and humidity condensate. Biological processes were used for primary degradation of organic material as well as for nitrification of ammonium in the wastewater. Physical-chemical systems removed inorganic salts from the water and provided post-treatment. The integrated system provided potable water to the crew throughout the test. This paper describes the water recovery system and reviews the performance of the system during the test.

The effects of both the cosmic ray heavy ion exposures and the intense trapped electron exposures are examined with respect to impact on cellular system survival on exterior spacecraft surfaces as well as at interior (shielded) locations for a sample mission to Jupiter’s moons. Radiation transport through shield materials and subsequent exposures are calculated with the established Langley heavy ion and electron deterministic codes. In addition to assessing fractional DNA single and double strand breaks, a variety of cell types are examined that have greatly differing radio-sensitivities. Finally, implications as to shield requirements for controlled biological experiments are discussed.

This paper provides an overview of the IMMWPS Integrated Ground Test Program, completed at the NASA Johnson Space Center (JSC) during October and November 2001. The JSC Crew and Thermal Systems Division (CTSD) has developed the IMMWPS orbital flight experiment to test the feasibility of a microbe-based water purifier for use in zero-gravity conditions. The IMMWPS design utilizes a Microbial Processor Assembly (MPA) inoculated with facultative anaerobes to convert organic contaminants in wastewater to carbon dioxide and biomass. The primary purpose of the ground test program was to verify functional operations and procedures. A secondary objective was to provide initial ground data for later comparison to on-orbit performance. This paper provides a description of the overall test program, including the test article hardware and the test sequence performed to simulate the anticipated space flight test program. In addition, a summary of significant results from the testing is provided.

In-situ resource utilization (ISRU) is an important part of current mission architectures for both a return to the Moon and the eventual human exploration of Mars. ORBITEC has developed and demonstrated an innovative direct energy processing approach for carbon-reduction of lunar and Martian regolith that can operate in a nearly closed-loop manner. Carbon-reduction of regolith produces oxygen and a variety of other useful products, including silicon, iron and glass ceramic materials. In addition, various ISRU propulsion technologies that utilize lunar and Martian resources have been developed and demonstrated. Work is also being conducted with the USDA on techniques to use biomass and waste materials to manufacture items such as shelters, furniture, filters and paper. Atmospheric carbon dioxide on Mars would be used to support the production of biomass in excess of life support needs to be used as the raw material to manufacture useful products on-site.

Controlling microbial growth and biofilm formation in spacecraft water-distribution systems is necessary to protect the health of the crew. Methods to decontaminate the water system in flight may be needed to support long-term missions. We evaluated the ability of iodine and ozone to kill attached bacteria and remove biofilms formed on stainless steel coupons. The biofilms were developed by placing the coupons in a manifold attached to the effluent line of a simulated spacecraft water-distribution system. After biofilms were established, the coupons were removed and placed in a treatment manifold in a separate water treatment system where they were exposed to the chemical treatments for various periods. Disinfection efficiency over time was measured by counting the bacteria that could be recovered from the coupons using a sonication and plate count technique. Scanning electron microscopy was also used to determine whether the treatments actually removed the biofilm.

The wonder of space exploration is a sure way to catch the attention of students of all ages, and space biology is one of many sciences critical to understanding the spaceflight environment. Many systems used in the past for space-to-classroom biology activities have required extensive crew time and material resources, making space-linked education logistically and financially difficult. The new Education Payload Operations Kit C (EPO Kit C) aims to overcome obstacles to space-linked education and outreach by dramatically reducing the resources required for educational activities in plant space biology that have a true spaceflight component. EPO Kit C is expected to be flown from STS-118 to the International Space Station in June 2007. NASA and several other organizations are currently planning an outreach program to complement the flight of EPO Kit C.

Biological water processors are currently being developed for application in microgravity environments. Work has been performed to develop a single-phase, gravity independent anoxic denitrification reactor for organic carbon removal [1]. As a follow on to this work it was necessary to develop a gravity independent nitrification reactor in order to provide sufficient nitrite and nitrate to the organic carbon oxidation reactor for the complete removal of organic carbon. One approach for providing the significant amounts of dissolved oxygen required for nitrification is to require the biological reactor design to process two-phase gas and liquid in micro-gravity. This paper addresses the design and test results overview for development of a tubular, two-phase, gravity independent nitrification biological water processor.