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ECOSIM, a software tool for the simulation of ECLSS which has been developed for the European Space Agency (ESA), provides a powerful, friendly graphical interface to users. To test and verify the evolutional capability of ECOSIM for the simulation of ECLSS for future missions, and also to clarify the required future improvements to ECOSIM, the ECLSS of a Moon base, in its initial build-up phase, has been modelled and simulated, following the establishment of the necessary new component mathematical models, including biological ones.