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Worldwide diesel fuels differ in their composition and therefore in thermo-physical properties. Some of these properties are known to have little effect on the combustion process. Others, like the cetane number, have dramatic influence on the combustion formation and thus on the heat release rate and more important the formation of soot and NOx. In an experiment series various commercially available fuel types, like EN 590 [1], ASTM D975 [2] and JIS K 2204 [3], have been compared to alternative diesel fuels such as FAME, GtL and premium diesel fuel with increased cetane number. A specially designed research injector was used in order to provide full optical access to one single fuel jet injected and combusted in a constant volume vessel. First, the liquid fuel phase propagation has been investigated by means of Mie-scattering and the liquid penetration depth and the spray cone angle have been evaluated.