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Due to the challenges in quantifying hand loads in manufacturing environments it is often assumed that the load is evenly distributed between the hands, even when handling parts with non-uniform mass distribution. This study estimated hand loads for six female subjects, when handling a custom part in 8 different configurations (2 weights, 4 CofM locations). The calculated hand loads varied from 20 to 50% of the weight being handled. The magnitude of asymmetrical hand loading depended on both the part orientation and the location of the CoM. Based on this study the knowledge of part weight, CofM location and hand positioning will allow the users of digital human models to perform more realistic and reliable task analysis assessments as the force distributions will be more representative of the actual loading rather than simply assuming the load is evenly distributed between the hands.