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Two crops of lettuce (Lactuca sativa cv. Waldmann's Green) were grown in the Regenerative Life Support Systems (RLSS) Test Bed at NASA's Johnson Space Center. The RLSS Test Bed is an atmospherically closed, controlled environment facility for the evaluation of regenerative life support systems using higher plants. The chamber encloses 10.6 m2 of growth area under cool-white fluorescent lamps. Lettuce was double seeded in 480 pots, each containing about 250 cm3 of calcined-clay substrate. Each pot was irrigated with half-strength Hoagland's nutrient solution at an average total applied amount of 2.5 and 1.8 liters pot-1, respectively, over each of the two 30-day crop tests. Average environmental and cultural conditions during both tests were 23°C air temperature, 72% relative humidity, 1000 ppm carbon dioxide (CO2), 16h light/8h dark photoperiod, and 356 μmol m-2s-1 photosynthetic photon flux.

The Thermoelectric Integrated Membrane Evaporative Subsystem (TIMES) was evaluated to determine its ability to process reverse osmosis brine as one of the Advanced Water Processor steps. As the baselined membrane (Nafion 117) was unable to retain ammonia, a NASA-sponsored project was conducted to find an alternate membrane capable of retaining ammonia while maintaining reasonable water production rates. A long term test was performed with up to 90% water recovery using a hydrophilic membrane and a hydrophobic membrane. The ammonia rejection for the hydrophilic membrane was 80%. The average water production rates for both membranes were greater than for the pretreated baselined Nafion membrane.