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This work studies the potential of ethanol-Biodiesel-Diesel fuel blends in both conventional Diesel and HCCI combustion modes. First, ethanol based fuels were tested on a modern commercial multi-cylinder DI diesel engine. The aim of this phase was to assess how such fuels affect Diesel engine performances and emissions. These results indicate that low levels of PM and NOx emissions, with a contained fuel consumption penalty and with an acceptable noise level, are achievable when the Diesel-ethanol blends are used in combination with an optimized combustion control. Moreover, experiments with ethanol based blends were performed using a single cylinder engine, running under both early injection HCCI and Diesel combustion modes. Compared to a conventional fuel, these blends allow increasing the HCCI operating range and also lead to higher maximum power output in conventional Diesel combustion.