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During 1995, we tested instruments and attempted a seed-to-seed experiment with Super-Dwarf wheat in the Russian Space Station Mir. Utah instrumentation included four IR gas analyzers (CO2 and H2O vapor, calculate photosynthesis, respiration, and transpiration) and sensors for air and leaf (IR) temperatures, O2, pressure, and substrate moisture (16 probes). Shortly after planting on August 14, three of six fluorescent lamp sets failed; another failed later. Plastic bags, necessary to measure gas exchange, were removed. Hence, gases were measured only in the cabin atmosphere. Other failures led to manual watering, control of lights, and data transmission. The 57 plants were sampled five times plus final harvest at 90 d. Samples and some equipment (including hard drives) were returned to earth on STS-74 (Nov. 20). Plants were disoriented and completely vegetative. Maintaining substrate moisture was challenging, but the moisture probes functioned well.

Liquid and gas exchange within a particulate plant-rooting medium is likely to be altered in a microgravity environment. A difference in gravitational force can result in significant offsets in control parameters developed on earth for optimum plant growth, due to the shift in hydrostatic water distribution. The experiment being developed will examine the effects of variable gravity on water distribution and gas diffusion. We are developing and testing an automated gas diffusion measurement system for use on the International Space Station (ISS). To allow comparison of μg and 1g conditions, gas diffusion cell designs were horizontally oriented to minimize gravitational effects using 1) a ‘thin rectangular profile’ cell and 2) a cylindrical cell design for flight. Electronic solenoid valves provide air and water flow control while pressure transducers measure water and substrate potential.