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The Rayleigh light scattering technique has been used to quantify the mean and fluctuating concentration of a passive scalar used to simulate fuel injection in a reciprocating, two-stroke model engine motored at 200 rpm in the absence of compression. The transient concentration field, which results from injection of Freon-12 vapour through the centre of an axisymmetrically located permanently open valve, has been investigated for injection timings of 40 deg. before and at top-dead-centre as a function of spatial position and crank angle. The purpose-built Rayleigh system, with gated digital data acquisition and software dust particle filtering, was first evaluated in a Freon-12 free jet by comparing results to those obtained with a sampling probe.