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The objectives of these experiments were two fold: 1) to determine if accepted tissue fixation procedures can be adapted for long-term storage on-board the International Space Station (ISS) and 2) to test whether they are suitable for KSC Fixation Tubes (KFTs). Three tissue fixation parameters were tested: time, temperature and sample size.Fixation time:samples are normally fixed from several minutes to several hours, but because of the duration of the UF-1 mission to ISS, the tissue will be in fixative for up to 3 months. Super dwarf wheat tissue samples were fixed in either FAA (a fixative normally used for storage of plant tissues) or a glutaraldehyde-based fixative and stored for 100 days.