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This paper addresses the effect of waste processing on advanced life support (ALS) system design. Waste processing is a critical component of an advanced life support system. It must take all life support system wastes and either convert them into useful products or into a form in which they can be discarded. Waste can be treated as soon as it is produced, stored as is, or processed into an intermediate form for further treatment. The decisions made will affect the cost-effectiveness of the system. Strategies must be developed to meet waste processing requirements for specific mission scenarios.

Bioregenerative resource recovery components for Advanced Life Support systems will need to be reliable and stable for long duration space travel. Since 1989, bioregenerative life support research at the ALS Breadboard Project has examined processing of inedible crop residues in bioreactors for recovery of nutrients for replenishment of crop hydroponic solutions. Bioreactor operation has been reliable as demonstrated by continuous operation for up to 418 days with long periods of steady state conditions. Bioreactors have demonstrated stability following unplanned, non-lethal perturbations in pH, temperature, dissolved oxygen, and inedible residue supply. In each instance, a rapid return to steady state conditions was observed.

The National Aeronautics and Space Administration (NASA) intends to continue the human exploration of outer space. Long duration missions will require the development of reliable regenerative life support processes. The intent of this paper is to define the Kennedy Space Center Controlled Ecological Life Support System (CELSS) research plan for the development and testing of three candidate biological processors for a hybrid biological and physical-chemical waste recycling system. The system would be capable of reclaiming from inedible plant biomass, human metabolic waste, and gray water those components needed for plant growth (carbon dioxide, water, and inorganic salts), while eliminating noxious compounds and maximizing system closure. We will colaborate with AMES Research Center (ARC), Johnson Space Center (JSC), and academia, to design a functional biological-based waste processing system that could be integrated with the planned Human Rated Test Facility (HRTF) at JSC.

Although bioprocessors have been successfully tested in ground test experiments as primary wastewater processors [1, 2 and 3], the transition required for operation of a bioprocessor in microgravity is complicated by the absence of gravity and buoyancy-driven convection. Gases are present in the wastewater bioprocessor from numerous sources including aeration, metabolic production and operation. This paper presents an innovative approach to the delivery of metabolically-required oxygen to a bioprocessor. A bioprocessor that provides oxygen delivery and bacterial support using membranes has been developed and tested during the past two years. Bench-top laboratory results have demonstrated that Total Organic Carbon (TOC) degradation above 95%, and nitrification above 80% can be maintained, while denitrification typically ranged between 5-25% in a membrane bioprocessor system (MBS).

The work presented in this paper summarizes the performance of subsystems used during an integrated advanced water recovery system test conducted by the Crew and Thermal Systems Division (CTSD) at NASA-Johnson Space Center (JSC). The overall objective of this test was to demonstrate the capability of an integrated advanced water recovery system to produce potable quality water for at least six months. Each subsystem was designed for operation in microgravity. The primary treatment system consisted of a biological system for organic carbon and ammonia removal. Dissolved solids were removed by reverse osmosis and air evaporation systems. Finally, ion exchange technology in combination with photolysis or photocatalysis was used for polishing of the effluent water stream. The wastewater stream consisted of urine and urine flush water, hygiene wastewater and a simulated humidity condensate.

The work presented in this paper summarizes the early results of an integrated advanced water recovery system test conducted by the Crew and Thermal Systems Division (CTSD) at NASA-Johnson Space Center (JSC). The system design and the results of the first two months of operation are presented. The overall objective of this test is to demonstrate the capability of an integrated advanced water recovery system to produce potable quality water for at least six months. Each subsystem is designed for operation in microgravity. The primary treatment system consists of a biological system for organic carbon and ammonia removal. Dissolved solids are removed by reverse osmosis and air evaporation systems. Finally, ion exchange technology in combination with photolysis or photocatalysis is used for polishing of the effluent water stream. The wastewater stream consists of urine and urine flush water, hygiene wastewater and a simulated humidity condensate.

Three Intermediate-Scale Aerobic Bioreactors were designed, fabricated, and operated. They utilized mixed microbial communities to bio-degrade plant residues. The continuously stirred tank reactors operated at a working volume of 8 L, and the average oxygen mass transfer coefficient, kLa, was 0.01 s-1. Mixing time was 35 s. An experiment using inedible wheat residues, a replenishment rate of 0.125 day-1, and a solids loading rate of 20 gdw day-1 yielded a 48% reduction in biomass. Bioreactor effluent was successfully used to regenerate a wheat hydroponic nutrient solution. Over 80% of available potassium, calcium, and other minerals were recovered and recycled in the 76-day wheat growth experiment.

Ground-based laboratory and closed-chamber human tests have demonstrated the ability of microbial-based biological processors to effectively remove carbon and nitrogen species from regenerable life support wastewater streams. Application of this technology to crewed spacecraft requires the development of gravity-independent bioprocessors due to a lack of buoyancy-driven convection and sedimentation in microgravity. This paper reports on the development and testing of membranebased biological reactors and addresses the processing of planetary and International Space Station (ISS) waste streams. The membranes provide phase separation between the wastewater and metabolically required oxygen, accommodate diffusion-driven oxygen transport, and provide surface area for microbial biofilm attachment. Testing of prototype membrane bioprocessors has been completed.

A Breadboard-Scale Aerobic Bioreactor (B-SAB) has been designed and integrated with the Kennedy Space Center's Biomass Production Chamber (BPC). The bioreactor utilizes a mixed microbial community to biodegrade inedible plant residues, a component of a Controlled Ecological Life Support System (CELSS) waste-stream. The continuously stirred tank reactor (120 L working volume) supports nutrient recycling and secondary food production experiments, and can process an influent with a solids loading as high as 50 g L-1. The volumetric oxygen mass transfer coefficient, kLa, is 0.013 s-1. Nutrient solution for BPC lettuce and wheat crops has been produced. Currently, B-SAB is supplying 80% of the nutrients for 10 m2 of potato plants in a continuous production experiment.

The KSC Breadboard Scale Aerobic Bioreactor (B-SAB) was used to bioprocess inedible wheat crop residues to provide recycled nutrients to support crop growth in the JSC Variable Pressure Growth Chamber (VPGC) as part of the 91 day JSC-Lunar/Mars Life Support Test Project Phase III. To meet the wheat nutrient demand at JSC, the KSCB-SAB was operated at both a higher loading rate (35 gdw L-1 compared with 20 gdw L-1) and at a slower retention time (21 days compared with 8 days) than we had used in previous bioreactor (continuous stirred tank reactor - CSTR) studies. The bioreactor operated for 19 weeks-8 weeks startup and steady state stabilization then 11 weeks of operation with the broth harvested weekly. Filtered broth was amended with nutrients and transported to JSC for integration into the VPGC wheat growth component of L/MLSTP Phase III. Biodegradation of JSC wheat residues was a constant 45% during steady state bioreactor operation, and similar to previous B-SAB runs.

The Biological Wastewater Processor Experiment Definition team is performing the preparatory ground research required to define and design a mature space flight experiment. One of the major outcomes from this work will be a unit-gravity prototype design of the infrastructure required to support scientific investigations related to microgravity wastewater bioprocessing. It is envisioned that this infrastructure will accommodate the testing of multiple bioprocessor design concepts in parallel as supplied by NASA, small business innovative research (SBIR), academia, and industry. In addition, a systematic design process to identify how and what to include in the space flight experiment was used.

Manned space missions require the development of compact, efficient, and reliable life support systems. A number of aqueous biological conversion processes are associated with bioregenerative life support systems. Vessels, or bioreactors, capable of supporting these processes in microgravity must be developed. An annular flow bioreactor has been conceived. It has the potential to incorporate containment, phase separation, gas exchange, and illumination into a single vessel. The bioreactor utilizes capillary fluid management techniques and is configured as a cylindrical tube in which a two-phase liquid-gas flow is maintained. Vanes placed around the inner perimeter enhance capillary forces and cause the liquid phase to attach and flow along the interior surface of the tube. No physical barrier is required to complete phase separation.