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Presented is a synopsis of ongoing research into the development of techniques and hardware required to produce useable quantities of astrobiology relevant biomass under controlled and repeatable laboratory conditions. This study has developed microbial habitats (referred to as digesters, due to their biomass production function) capable of sustaining microbial communities under low temperature, high salt, high sulfate, anaerobic conditions. This set of basic conditions is necessary to develop biomass material that is analog to the biomass that would be present in subsurface brine habitats on Mars or Europa, from the perspective of several critical biochemical properties.

One of the key questions addressed in the field of Astrobiology is based upon the assumption that life exists, or at one time existed, in locations throughout the universe. However, this assumption is just that, an assumption. No definitive proof exists. On Earth, life has been found to exist in many diverse environments. We believe that this tendency towards diversity supports the assumption that life could exist wherever environmental conditions are right to support it. There are several locations within our Solar System which might support environments which are conducive to life. However, to conclusively establish the existence of life in such locations we must be capable of sensing generic life forms. This paper provides a summary of several innovative techniques based on the use of fluorescent analysis for the direct detection of extraterrestrial life forms.

The objective of this work was to determine if chemoautotrophic organisms exist in the vicinity of hydrothermal vents, specifically organisms that have the capability to utilize non-solar electromagnetic radiation. The discriminating criteria used to make this determination was the existence of, or lack of, chlorophyllic compounds in these organisms. Work conducted to this point indicates that pigment containing microbial species does exist within the hydrothermal vent microenvironment. Furthermore, it does appear that at least some of these pigments are bacteiochlorophylls. A question still remains unanswered, that is, are these organisms indigenous to the vent community or debris from surface environments?

The study of life in extreme environments provides an important basis from which we can undertake the search for extraterrestrial life. This paper provides a description of a program focused on developing technologies which are necessary to evaluate the potential for the existence of a deep sub-seafloor biosphere.

This paper compares four potential water treatment systems in the context of their applicability to a Mars transit vehicle mission. The systems selected for evaluation are the International Space Station system, a JSC bioreactor-based system, the vapor phase catalytic ammonia removal system, and the direct osmotic concentration system. All systems are evaluated on the basis of their applicability for use in the context of the Mars Reference Mission. Each system is evaluated on the basis of mass equivalency. The results of this analysis indicate that there is effectively no difference between the International Space Station system and the JSC bioreactor configurations. However, the vapor phase catalytic ammonia removal and the direct osmotic concentration systems offer a significantly lower mass equivalency (approximately 1/7 the ISS or bioreactor systems).