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In this work, two blends of isopropanol, n-butanol, and ethanol (IBE) that can be produced by metabolically engineered clostridium acetobutylicum are studied experimentally in advanced compression ignition (ACI). This is done to determine whether these fuel blends have the right fuel properties to enable thermally stratified compression ignition, a stratified ACI strategy that using the cooling potential of single stage ignition fuels to control the heat release process. The first microorganism, ATCC824, produces a blend of 34.5% isopropanol, 60.1% n-butanol, and 5.4% ethanol, by mass. The second microorganism, BKM19, produces a blend of 12.3% isopropanol, 54.0% n-butanol, and 33.7% ethanol, by mass. The sensitivity of both IBE blends to intake pressure, intake temperature, and cylinder energy content (fueling rate) is characterized and compared to that of its neat constituents. Both IBE blends behaved similarly with a reactivity level between that of ethanol and n-butanol.

In this work, a novel opposed piston architecture is proposed where one crankshaft rotates at twice the speed of the other. This results in one piston creating a 2-stroke profile and another with a 4-stroke profile. In this configuration, the slower piston operates in the 2-stroke CAD domain, while the faster piston completes 2 reciprocating cycles in the same amount of time (4-stroke). The key benefit of this cycle is that the 4-stroke piston increases the rate of compression and expansion (dV/dθ), which lowers the combustion-induced pressure rise rate after top dead center (crank angle location of minimum volume). Additionally, it lowers in-cylinder temperatures and pressures more rapidly, resulting in a lower residence time at high temperatures, which reduces residence time for thermal NOx formation and reduces the temperature differential between the gas and the wall, thereby reducing heat transfer.