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A system was developed which provides nutrients and water to plants while maintaining good aeration at the roots and preventing water from escaping in reduced gravity. The nutrient solution is circulated through porous tubes under negative pressure and moves through the tube wall via capillary forces into the rooting matrix, establishing a non-saturated condition in the root zone. Tests using prototypes of the porous tube water and nutrient delivery system indicate that plant productivity in this system is equivalent to standard soil and solution culture growing procedures. The system has functioned successfully in short-term microgravity during parabolic flight tests and will be flown on the space shuttle. Plants are one of the components of a bioregenerative life support system required for long duration space missions.

The Advanced Animal Habitat (AAH) represents the next generation of Space Station based animal research facilities. Building upon previously developed flight hardware and experience, the AAH offers greatly enhanced system capabilities and performance. The design focuses upon the creation of a robust and flexible platform capable of supporting present and future experimental needs. A modular packaging and distributed control architecture leads to increased system adaptability and expandability. The baseline configuration includes group housing capability for up to six rats with automated food and water delivery as well as waste collection. Animals are continuously monitored with three cameras during both day and night cycles. The animals can be accessed while on-orbit through the Life Sciences Glovebox to perform a wide variety of experimental protocols.

The Biomass Production System (BPS) was flown on the ISS for 73 days as part of the Increment 4 mission. To obtain maximum benefit from the long mission duration, numerous manual crew procedures were incorporated into the BPS experiments. These procedures included gas sampling, root module priming, harvesting, pollination, filter cleaning, water refill, and water sampling. On-orbit crew assessments were filled out for each of these procedures to evaluate the ability of BPS to accommodate them. The assessment asked questions about each phase of an activity and solicited recommendations for improvements. Further analysis of most procedures was provided by detailed video made on-orbit and multiple post-flight crew debriefs. Most assessments indicated no need for improvements, but a number of crew suggestions will be incorporated into hardware and procedure updates.

The ASTROCULTURE™ (ASC) middeck flight experiment series was developed to test and integrate subsystems required to grow plants in reduced gravity, with the goal of developing a plant growth unit suitable for conducting quality biological research in microgravity. Flights on the Space Shuttle have demonstrated control of water movement through a particulate rooting material, growth chamber temperature and humidity control, LED lighting systems and control, recycling of recovered condensate, ethylene scrubbing, and carbon dioxide control. A complete plant growth unit was tested on STS-63 in February 1995, the first ASC flight in which plant biology experiments were conducted in microgravity. The methods and objectives used for control of environmental conditions in the ASC unit are described in this paper.

The ASTROCULTURE™ (ASC) middeck flight experiment series was developed to test subsystems required to grow plants in reduced gravity, with the goal of developing a plant growth unit suitable for conducting quality biological research in microgravity. Previous Space Shuttle flights (STS-50 and STS-57) have successfully demonstrated the ability to control water movement through a particulate rooting matrix in microgravity and the ability of LED lighting systems to provide high levels of irradiance without excessive heat build-up in microgravity. The humidity and temperature control system used in the middeck flight unit is described in this paper. The system controls air flow and provides dehumidification, humidification, and condensate recovery for a plant growth chamber volume of 1450 cm3.

Fuel-borne catalysts (FBC) have demonstrated efficacy as an important strategy for integrated diesel emission control. The research summarized herein provides new methodologies for the characterization of engine-out speciated emissions. These analytical tools provide new insights on the mode of action and chemical forms of metal emissions arising from use of a platinum and cerium based commercial FBC, both with and without a catalyzed diesel particulate filter. Characterization efforts addressed metal solubility (water, methanol and dichloromethane) and particle size and charge of the target species in the water and solvent extracts. Platinum and cerium species were quantified using state-of-the-art high resolution plasma mass spectrometry. Liquid-chromatography-triple quad mass spectrometry techniques were developed to quantify potential parent Pt-FBC in the PM extracts. Speciation was examined for emissions from cold and warm engine cycles collected from an engine dynamometer.

The demand for highly flexible manipulation of plant growth generations, modification of specific plant processes, and genetically engineered crop varieties in a controlled environment has led to the development of a Commercial Plant Biotechnology Facility (CPBF). The CPBF is a quad-middeck locker playload to be mounted in the EXPRESS Rack that will be installed in the International Space Station (ISS). The CPBF integrates proven ASTROCULTURE” technologies, state-of-the-art control software, and fault tolerance and recovery technologies together to increase overall system efficiency, reliability, robustness, flexibility, and user friendliness. The CPBF provides a large plant growing volume for the support of commercial plant biotechnology studies and/or applications for long time plant research in a reduced gravity environment.

The CANDS (Circulating, Aeration, and Nutrient Delivery System) Phase II SBIR is currently developing and testing methods and procedures to control moisture, oxygen, and temperature in the root zone of a particulate based micro-gravity nutrient delivery system. The completion of the first year and a half of the CANDS Phase II SBIR has shown significant engineering developments towards environmental control of the root zone. These developments include the measurement of root zone oxygen content, characterization of forced and flood-ebb aeration rates, successful control of root zone moisture using miniature heat-pulse moisture sensors, and successful control of root zone temperature via an insulating/temperature controlling water jacket. At the conclusion of the CANDS Phase II SBIR an integrated root zone environmental control system will be constructed for integration into plant growth systems to eliminate the uncertainties that exist in current plant growth data.

A modular and scalable Dense Medium Plasma Water Purification Reactor was developed, which uses atmospheric-pressure electrical discharges under water to generate highly reactive species to break down organic contaminants and microorganisms. Key benefits of this novel technology include: (i) extremely high efficiency in both decontamination and disinfection; (ii) operating continuously at ambient temperature and pressure; (iii) reducing demands on the containment vessel; and (iv) requiring no consumables. This plasma based technology was developed to replace the catalytic reactor being used in the planned International Space Station Water Processor Assembly.