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The influence of exhaust gas recirculation (EGR) on the formation of nitric oxide (NO) was studied experimentally in a transparent gasoline direct injection engine by quantitative laser-induced fluorescence imaging. Spectral properties of the excited transition within the NO A2∑+-X2∏(0,2) band are well known from previous studies. The excitation scheme allows quantitative NO concentration measurements without detailed knowledge of the gas phase temperature. Good agreement was found with exhaust gas NOx chemi-luminescence (CLD) measurements. The experiments were carried out in an optically accessible gasoline engine featuring a direct injection cylinder head (BMW) and a Bosch injection system, based on a serial inline six-cylinder engine with an enlarged crankcase. The measurements were performed in the pentroof section of the combustion chamber.