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Heavy-duty transportation is one of the sectors that contributes to greenhouse gas emissions. One way to reduce CO2 emissions is to use drop-in fuels. However, when drop-in fuels are used, i.e., higher blends of alternative fuels are added to conventional fuels, solubility problems and precipitation in the fuel can occur. As a result, insolubles in the fuel can clog the fuel filters and interfere with the proper functioning of the injectors. This adversely affects engine performance and increases fuel consumption. These problems are expected to increase with the development of more advanced fuel systems to meet upcoming environmental regulations. This work investigates the composition of the deposits formed inside the injectors of the heavy-duty diesel engine and discusses their formation mechanism. Injectors with internal deposits were collected from field trucks throughout Europe. Similar content, location and structure were found for all the deposits in the studied injectors.