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An experimental study was performed to investigate the effects of various intake charge motion control valves (CMCVs) on mixture preparation, combustion, and hydrocarbon (HC) emissions during the cold start-up process of a port fuel injected spark ignition (SI) engine. Different charge motions were produced by three differently shaped plates in the CMCV device, each of which blocked off 75% of the engine's intake ports. Time-resolved HC, CO and CO2 concentrations were measured at the exhaust port exit in order to achieve cycle-by-cycle engine-out HC mass and in-cylinder air/fuel ratio. Combustion characteristics were examined through a thermodynamic burn rate analysis. Cold-fluid steady state experiments were carried out with the CMCV open and closed. Enhanced charge motion with the CMCV closed was found to shorten the combustion duration, which caused the location of 50% mass fraction burned (MFB) to occur up to 5° CA earlier for the same spark timing.