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The measurement of brake dust particles is a complex challenge owing to its open system configuration; indeed, the emitted particles are directly spread into the environment. Measurements on the inertia brake dyno feature controllable and reproducible environmental and operational parameters. Although Real Driving Emission (RDE) measurements enable the detection of brake dust particles emitted in real driving conditions (i.e. traffic condition, driving style, air humidity, vehicle components’ wear and ageing, etc.), they are complex and not reproducible due to external, continuously changing parameters (e.g., flow conditions, changing traffic conditions, particulate matter from other sources). The motivation lies in developing a real driving emission sampling system for brake particle emissions, which meets the quality requirements of the measurements, as well as the prevention of particle losses and contamination, thereby supplementing and reviewing laboratory-based procedures.