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As one of the pollutants that cannot be ignored, soot has a great impact on human health, environment, and energy conversion. In this investigation, the effect of residence time (25ms, 35ms, and 45ms) and ammonia on morphology and nanostructure of soot in laminar ethylene flames has been studied under atmospheric conditions and different flame heights (15 mm and 30 mm). The transmission electron microscopy (TEM) and high-resolution transmission electron microscope (HRTEM) are used to obtain morphology of aggregates and nanostructure of primary particles, respectively. In addition, to analyze the nanostructure of the particles, an analysis program is built based on MATLAB software, which is able to obtain the fringe separation distance, fringe length, and fringe tortuosity parameters of primary particles, and has been verified by the multilayer graphene interlayer distance.